کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1364152 | 981530 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
[35S]GTPγS binding studies of amphiphilic drugs-activated Gi proteins: A caveat
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: [35S]GTPγS binding studies of amphiphilic drugs-activated Gi proteins: A caveat [35S]GTPγS binding studies of amphiphilic drugs-activated Gi proteins: A caveat](/preview/png/1364152.png)
چکیده انگلیسی
This paper documents a serious problem met during the testing of Gi protein-activating properties of a new series of synthetic compounds by measuring the induced binding of [35S]GTPγS to different subtypes of Gi protein. The problem arose from the strong affinity between [35S]GTPγS and the tested compounds, that are characterized by several (2–4) positive charges and high lipophilicity. Apparently, such affinity yields insoluble, labelled complexes that, also in the absence of Gi protein, are retained on the filters and give rise to false positive results.
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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 19, Issue 8, 15 April 2009, Pages 2224–2229
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 19, Issue 8, 15 April 2009, Pages 2224–2229
نویسندگان
Dina Manetti, Lorenzo Di Cesare Mannelli, Silvia Dei, Luca Guandalini, Elisabetta Martini, Martina Banchelli, Carla Ghelardini,