کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1364564 | 981540 | 2008 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Correlation of inhibitor effects on enzyme activity and thermal stability for the integral membrane protein fatty acid amide hydrolase
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
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چکیده انگلیسی
The melting curves of fatty acid amide hydrolase (FAAH) in the presence of 29 reversible inhibitors were measured using a thiol-reactive fluorophore. The thermal stability (Tm) of the FAAH/inhibitor complex varied significantly depending on the chemical characteristics of the inhibitors, notably variations in the head group. Two separate distributions were observed when Tm was plotted against Ki. The majority of the inhibitors showed a positive correlation between binding affinity and Tm, however inhibitors with a pyridine carboxylic acid moiety in the head group fell in a distinct and uncorrelated distribution when tail groups were varied.
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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 18, Issue 22, 15 November 2008, Pages 5847–5850
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 18, Issue 22, 15 November 2008, Pages 5847–5850
نویسندگان
Ian M. Slaymaker, Michael Bracey, Mauro Mileni, Joie Garfunkle, Benjamin F. Cravatt, Dale L. Boger, Raymond C. Stevens,