Synthesis and characterization of BODIPY-labeled colchicine

Two BODIPY-labeled colchicine derivatives were synthesized and shown to bind to tubulin but only partially inhibit tubulin polymerization in the presence of GTP. Cytotoxicity studies were carried out in HeLa, HepG2, Raji and Vero cells. Apoptosis-inducing properties were determined by caspase 3/7 activity and flow cytometry and interactions between the derivatives and tubulin were verified by fluorescence microscopy of living cells.

Photo-stable and pH-independent BODIPY-labeled colchicine analogs were synthesized to allow analysis of the cellular distribution of tubulin.Figure optionsDownload as PowerPoint slide