کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1364585 981540 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of bestatin-based activity-based probes for metallo-aminopeptidases
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Development of bestatin-based activity-based probes for metallo-aminopeptidases
چکیده انگلیسی

A novel set of activity-based probes (ABPs) for functionally profiling metallo-aminopeptidases was synthesized based on the bestatin inhibitor scaffold, the first synthesis of bestatin analogues using solid-phase techniques. These ABPs were shown to label metallo-aminopeptidases, using both a biotin and a fluorophore reporter, in an activity-dependent manner. This probe class was also shown to be amenable to ‘click’ chemistry labeling for possible use in live cells. Finally, we demonstrate that the ABPs are able to label an aminopeptidase in a complex proteome. Thus, these bestatin-based probes should have wide utility to functionally profile aminopeptidases in many biological systems.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 18, Issue 22, 15 November 2008, Pages 5932–5936
نویسندگان
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