کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1364586 981540 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phage display of functional, full-length human and viral membrane proteins
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Phage display of functional, full-length human and viral membrane proteins
چکیده انگلیسی

Phage display of protein and peptide libraries offers a powerful technology for the selection and isolation of ligands and receptors. To date, the technique has been considered limited to soluble, non-membrane proteins. We report two examples of phage display of full-length, folded and functional membrane proteins. Consistent display required the recently reported KO7+ helper phage. The two proteins, full-length caveolin-1 and HIV gp41, display well on the surface of the phage, and maintain their binding activities as shown by in vitro assays.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 18, Issue 22, 15 November 2008, Pages 5937–5940
نویسندگان
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