Construction of saccharide-modified DNAs by DNA polymerase

Novel deoxyribonucleotide triphosphates bearing maltose or lactose groups were synthesized as substrates for DNA polymerase. The incorporation efficiencies of these modified substrates were investigated in both primer extension reactions and PCR. The stability and conformation of saccharide-modified dsDNAs were assessed by UV absorbance melting experiments and CD analysis. Enzymatic incorporation of saccharide-modified substrates can be used for the efficient production of saccharide-modified DNAs.

The construction of saccharide-modified DNAs using saccharide-modified dUTPs and DNA polymerase and the characterization of their biophysical properties are reported.Figure optionsDownload as PowerPoint slide