کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1367084 | 981615 | 2007 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Screening of α-helical peptide ligands controlling a calcineurin-phosphatase activity Screening of α-helical peptide ligands controlling a calcineurin-phosphatase activity](/preview/png/1367084.png)
In this paper, we describe an application of 202-membered fluorescently labeled peptide library designed to take an α-helix secondary structure. As a proof-of-concept experiment, a calmodulin (CaM)/calcineurin (Cn) pair was chosen to screen α-helical peptide ligands that tightly bind to CaM and also control enzymatic functions of Cn. Three peptides were successfully selected from the library by assaying Cn-phosphatase activities and peptide–CaM interactions (dual check process). The strategy using a designed peptide library shows real promise as a peptide-based high-throughput screening system.
Demonstrated was the application of screening for peptide ligands that tightly bind to a target protein and also control the protein’s functions. The strategy using a designed peptide library shows real promise as a peptide-based novel screening system.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 17, Issue 1, 1 January 2007, Pages 167–171