کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1368547 | 981701 | 2016 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Azadirachta indica triterpenoids promote osteoblast differentiation and mineralization in vitro and in vivo Azadirachta indica triterpenoids promote osteoblast differentiation and mineralization in vitro and in vivo](/preview/png/1368547.png)
Terpenoids were isolated using chromatographic purification through solvent purification technique and identified as Azadirone (1), Epoxyazadiradione (2) Azadiradione (3) Gedunin (4) Nimbin (5) Salannin (6) Azadirachtin A (7) and Azadirachtin B (8) from Azadirachta indica. Out of eight compounds, only three compounds had osteogenic activity and enhanced osteoblast proliferation, differentiation and mineralization in osteoblast cells. Active compounds stimulated osteogenic genes ALP, RunX-2 and OCN expressions in vitro, but Azadirachtin A had a maximum ability to stimulate osteoblast differentiation and mineralization compared to other two active compounds. For in vivo study, Azadirachtin A injected subcutaneously in pups, which enhanced osteogenic gene expressions and promoted bone formation rate significantly. Here, we conclude that active compounds of Azadirachta indica have osteogenic activity and Azadirachtin A has a beneficial effects on bone.
Schematic diagram outlining the novel triterpenoids isolated from Azadirachta indica by chromatographic techniques, have osteogenic activity. Out of eight compounds, only three compounds Azadirone, Azadirachtin A and Azadirachtin B have osteoblast proliferation, differentiation and mineralization ability. Azadirachtin A is most promising molecule which enhances gene expression and bone formation in vivo.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 26, Issue 15, 1 August 2016, Pages 3719–3724