کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1369041 | 981744 | 2014 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Improvement of a streptavidin-binding aptamer by LNA- and α-l-LNA-substitutions Improvement of a streptavidin-binding aptamer by LNA- and α-l-LNA-substitutions](/preview/png/1369041.png)
Forty modified versions of a streptavidin-binding aptamer each containing single or multiple LNA or α-l-LNA-substitutions were synthesized and their dissociation constants determined by surface plasmon resonance experiments. Both full-length and truncated versions of the aptamer were studied and compared with the unmodified DNA aptamers. A ∼two-fold improvement in binding affinity was achieved by incorporation of LNA nucleotides in the 3′-part of the stems of the streptavidin-binding aptamer whereas LNA- and α-l-LNA-substitutions in the terminal stem increased the serum stability.
A ∼two-fold improvement in binding affinity was achieved by incorporation of LNA nucleotides in the 3′-part of the stems of the streptavidin-binding aptamer whereas LNA- and α-l-LNA-substitutions in the terminal stem increased the serum stability.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 24, Issue 10, 15 May 2014, Pages 2273–2277