کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1370544 981822 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis of novel cationic spermine-conjugated phosphotriester oligonucleotide for improvement of cell membrane permeability
ترجمه فارسی عنوان
سنتز جدیدی از الیگونونوکلئوتید فسفودی اسیدهای اسپکتروفتامین برای بهبود نفوذ پذیری غشای سلولی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
چکیده انگلیسی

A spermine-conjugated ethyl phosphotriester oligonucleotide was obtained by solid-phase synthesis based on phosphoramidite chemistry. The ethyl phosphotriester linkage was robust to exonuclease digestion and stable in fetal bovine serum. Cell membrane permeability of the spermine-conjugated ethyl phosphotriester oligonucleotide was studied by fluorescence experiments. The effective cell penetrating potency of the spermine-conjugated ethyl phosphotriester oligonucleotide was determined by confocal laser scanning microscopy and measurement of intracellular fluorescence intensity.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 25, Issue 17, 1 September 2015, Pages 3610–3615
نویسندگان
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