Construction of a 19F-lectin biosensor for glycoprotein imaging by using affinity-guided DMAP chemistry

In this study, assisted by affinity-guided DMAP strategy, we developed a novel 19F-modified lectin as a biosensor for specific detection and imaging of glycoproteins. Exploited the large chemical shift anisotropy property of 19F nuclei, glycoproteins detected by our 19F-biosensor are signatured by broadened peaks in 19F NMR, hence enabled the distinction between glycoproteins and small molecule saccharides. Such signal on/off switching was also applied to glycoprotein imaging by 19F MRI.

Figure optionsDownload as PowerPoint slide