کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1373196 | 981893 | 2007 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Microbial transformation of three bufadienolides by Nocardia sp. and some insight for the cytotoxic structure–activity relationship (SAR) Microbial transformation of three bufadienolides by Nocardia sp. and some insight for the cytotoxic structure–activity relationship (SAR)](/preview/png/1373196.png)
Resibufogenin, cinobufagin, and bufalin are cytotoxic steroids isolated from the Chinese drug Chan’su. Biotransformation of these three bufadienolides by Nocardia sp. NRRL 5646 was investigated. Notably, resibufogenin was converted to 3-acetyl 15β-hydroxyl bufotalin, via an unprecedented 14β,15β-epoxy ring cleavage and a regio-selective acetoxylation. This product showed significantly increased cytotoxic activity. The regio-selective acetylation of the 3-OH was also involved in the other reactions. The structures of metabolites were established by ESI-LC/MS and 2D NMR techniques. The in vitro cytotoxic activities against human cancer cell lines of the substrates and the transformed products were determined by the MTT method and their structure–activity relationship (SAR) was discussed. This investigation provided a useful approach to prepare new bufadienolides and the SAR research.
Resibufogenin (1) was converted to a bufatalin derivate, 3-acetyl 15β-hydroxyl bufatalin (3), by Nocardia sp. NRRL 5646 through a selective epoxy ring cleavage and a regio-selective acetoxylation, the product showed significantly increased cytotoxicities.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 17, Issue 22, 15 November 2007, Pages 6062–6065