کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1373374 1500544 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel insights into the use of Glowing LNA as nucleic acid detection probes—Influence of labeling density and nucleobases
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Novel insights into the use of Glowing LNA as nucleic acid detection probes—Influence of labeling density and nucleobases
چکیده انگلیسی

Appropriately designed 2′-N-(pyren-1-yl)carbonyl-2′-amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 20, Issue 24, 15 December 2010, Pages 7265–7268
نویسندگان
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