A reverse transcriptase stop assay revealed diverse quadruplex formations in UTRs in mRNA

Here, we developed a reverse transcriptase based method (RTase stop assay) to characterize quadruplex formations in guanine-rich RNAs with high sensitivity and specificity. By using the RTase stop assay, we also revealed a plausible structural polymorphism in biologically important RNAs. The RTase stop assay would provide helpful insight into RNA quadruplex structures and functions, together with other analytical methods, including various footprinting techniques.

We developed an enzyme-based method to certify quadruplex formations in biologically important RNAs.Figure optionsDownload as PowerPoint slide