کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1374857 981927 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A method for terminus proteomics: Selective isolation and labeling of N-terminal peptide from protein through transamination reaction
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
A method for terminus proteomics: Selective isolation and labeling of N-terminal peptide from protein through transamination reaction
چکیده انگلیسی

A novel method for selectively labeling and isolating N-terminal peptide from protein has been developed. An Nα-amino group of protein was converted to a carbonyl group through transamination reaction and the resulting carbonyl group was modified with O-(4-nitrobenzyl)hydroxylamine (NBHA). After proteolytic digestion using Grifola frondosa metalloendopeptidase (LysN), the modified N-terminal peptide remained unbound in the following treatment using amino-reactive p-phenylenediisothiocyanate (DITC) glass, whereas peptides other than the N-terminal peptide were effectively scavenged from the supernatant solution. The modified N-terminal peptide was thus successfully isolated and sequenced by matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI-MS/MS) analysis.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 19, Issue 23, 1 December 2009, Pages 6544–6547
نویسندگان
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