کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1375033 981931 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
CSI–FID: High throughput label-free detection of DNA binding molecules
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
CSI–FID: High throughput label-free detection of DNA binding molecules
چکیده انگلیسی

Determining the sequence specifity of DNA binding molecules is a non-trivial task. Here we describe the development of a platform for assaying the sequence specificity of DNA ligands using label free detection on high density DNA microarrays. This is achieved by combining Cognate Site Identification (CSI) with Fluorescence Intercalation Displacement (FID) to create CSI–FID. We use the well-studied small molecule DNA ligand netropsin to develop this high throughput platform. Analysis of the DNA binding properties of protein- and small molecule-based libraries with CSI–FID will advance the development of genome-anchored molecules for therapeutic purposes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 19, Issue 14, 15 July 2009, Pages 3779–3782
نویسندگان
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