کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1375569 981941 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
β-Lactam-based approach for the chemical programming of aldolase antibody 38C2
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
β-Lactam-based approach for the chemical programming of aldolase antibody 38C2
چکیده انگلیسی

Irreversible chemical programming of monoclonal aldolase antibody (mAb) 38C2 has been accomplished with β-lactam-equipped targeting modules. A model study was first performed with β-lactam conjugated to biotin. This conjugate efficiently and selectively modified the catalytic site lysine (LysH93) of mAb 38C2. We then conjugated a β-lactam to a cyclic-RGD peptide to chemically program mAb 38C2 to target integrin receptors αvβ3 and αvβ5. The chemically programmed antibody bound specifically to the isolated integrin receptor proteins as well as the integrins expressed on human melanoma cells. This approach provides an efficient and versatile solution to irreversible chemical programming of aldolase antibodies.

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 19, Issue 5, 1 March 2009, Pages 1421–1424
نویسندگان
, , ,