کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1376351 | 981956 | 2008 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Oligonucleotide microarrays with stem–loop probes: Enhancing the hybridization of nucleic acids for sensitive analysis Oligonucleotide microarrays with stem–loop probes: Enhancing the hybridization of nucleic acids for sensitive analysis](/preview/png/1376351.png)
We have demonstrated that the dynamics of nucleic acid hybridization in microarrays depend on the physical structure of immobilized probes. We have immobilized oligonucleotide-3′-phosphates with and without stem–loop structure on epoxylated glass surface, followed by hybridization under different conditions, viz., hybridization buffer, pH condition, temperature and ionic strength. In a comparative study, we have established that array constructed using probes with stem–loop structure displayed ∼2.2 times higher hybridization signals than the probes without it. The stem–loop DNA array format is simple and flexible in design and thus potentially useful in various DNA diagnostic tests.
Here, we have demonstrated that the dynamics of nucleic acid hybridization in microarrays depend on the physical structure of the immobilized species.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 18, Issue 12, 15 June 2008, Pages 3585–3588