A graftable LDV peptidomimetic: Design, synthesis and application to a blood filtration membrane

A graftable LDV (Leu-Asp-Val) peptidomimetic molecule (B-c) has been prepared from 3-(5-amino-2-hydroxy)phenyl-propionic acid, as α4β1 (VLA-4) integrin ligand. For that purpose, the mechanism of 3-(4-azidophenyl)propionic acid rearrangement has been revisited. Activation of Durapore DVPP-hydrophilic membrane, by surface wet chemistry using triazine trifluoride, followed by covalent coupling of B-c produced a modified filter (0.8% of derivatisation from XPS analysis) with improved capacity of leukocyte retention.

A PVDF hydrophilic membrane was surface modified with a non-peptide mimic of the fibronectin LDV sequence designed as VLA-4 integrin ligand. Leukocyte depletion with such a filter was significantly improved.Figure optionsDownload as PowerPoint slide