کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1377352 | 981976 | 2008 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Cy3-3-acylcholine: A fluorescent analogue of acetylcholine for single molecule detection Cy3-3-acylcholine: A fluorescent analogue of acetylcholine for single molecule detection](/preview/png/1377352.png)
We synthesized a novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine. The molecular weight of the products agreed with structural predictions. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nAChR were visualized by TIRF microscopy. The agonist effect of the Cy3-3-acylcholine on nicotinic acetylcholine receptor (nAChR) was confirmed electrophysiologically. This newly synthesized fluorescent analogue will enable us to conduct more elaborate studies on single channel interaction processes between nAChR and ligands.
A novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine, was synthesized. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nicotinic acetylcholine receptors were visualized under microscopy.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 18, Issue 3, 1 February 2008, Pages 1106–1109