کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1377979 | 981992 | 2007 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A new visual screening assay for catalytic antibodies with retro-aldol retro-Michael activity A new visual screening assay for catalytic antibodies with retro-aldol retro-Michael activity](/preview/png/1377979.png)
Fast and convenient methods are required for the detection of novel catalysts. We have developed a new assay to allow direct visualization of retro-aldol retro-Michael catalytic activity and have demonstrated it with catalytic antibody 38C2. The assay is based on a catalytic cleavage of a physiologically stable substrate to release 3,4-cyclohexeneoesculetin. The latter then reacts with iron(III) to generate a non-soluble complex that precipitates in the form of a black dye. This assay may be used for screening new catalysts for retro-aldol retro-Michael activity with improved efficiency for specific prodrug activation.
A black precipitate that is formed of three molecules of 3,4-cyclohexenoesculetin per ion of iron III is applied for visual detection of retro-aldol retro-Michael catalytic activity.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 17, Issue 5, 1 March 2007, Pages 1172–1175