Radical scavenging activity of protein from tentacles of jellyfish Rhopilema esculentum

In this study, radical scavenging activity of protein from tentacles of jellyfish Rhopilema esculentum (R. esculentum) was assayed including superoxide anion radical and hydroxyl radical scavenging. The protein samples showed strong scavenging activity on superoxide anion radical and values EC50 of full protein (FP), first fraction (FF), second fraction (SF), and 30% (NH4)2SO4 precipitate (Fr-1) were 2.65, 7.28, 1.10, and 22.51 μg/mL, respectively, while values EC50 of BHA, BHT, and α-tocopherol were 31, 61, and 88 μg/mL, respectively. Also, the protein samples had strong scavenging effect on hydroxyl radical and the values EC50 of FP, FF, SF, Fr-1, and Fr-2 were 48.91, 27.72, 1.82, 16.36, and 160.93 μg/mL, but values EC50 of Vc and mannitol were 1907 and 4536 μg/mL, respectively. Of the five protein samples, SF had the strongest radical scavenging activity and may have a use as a possible supplement in the food and pharmaceutical industries. The radical scavenging activity was stable at high temperature so that R. esculentum may be used as a kind of natural functional food.

Radical scavenging activity of protein from tentacles of jellyfish Rhopilema esculentum by (NH4)2SO4 fractionation and Sephadex G-100 chromatography was assayed. The protein samples showed strong scavenging activity on superoxide anion and hydroxyl radical and the radical scavenging activity was stable at high temperature.