Synthesis and characterization of silver nanoparticles using crystal compound of sodium para-hydroxybenzoate tetrahydrate isolated from Vitex negundo. L leaves and its apoptotic effect on human colon cancer cell lines

• Sodium para-hydroxybenzoate tetrahydrate crystal compound was isolated and identified by single crystal X-ray diffraction.
• Synthesis and characterization of SPHT-AgNPs were confirmed by different spectroscopic and microscopic methods.
• Antiproliferative effect of SPHT and SPHT-AgNPs on human colon cancer cell lines.
• SPHT and SPHT-AgNPs induce apoptosis and abolish cell growth on cancer cell lines.

Metallic nanoparticles are major concern, particularly silver nanoparticles (AgNPs) are used in various applications. In the present investigation, we report a novel strategy with biological approach for synthesis of AgNPs using sodium para-hydroxybenzoate tetrahydrate (SPHT) isolated from Vitex negundo leaves. The synthesized SPHT-AgNPs were characterized by UV–vis spectroscopy, high resolution transmission electron microscopy (HRTEM) with selected area electron diffraction (SAED) pattern, field emission scanning electron microscopy (FESEM) with energy-dispersive X-ray spectroscopy (EDX), zeta potential and Fourier transform infrared spectroscopy (FT-IR) analysis. The various pH and temperature were evaluated to find their stability effects on SPHT-AgNPs synthesis peak at 430 nm. The size of SPHT-AgNPs were ranging from 26 to 39 nm and were spherical in shape. The hydroxyl and carboxylic functional groups from bio-reducing mediators of SPHT have a stronger ability towards synthesis of AgNPs, which was confirmed using FT-IR spectrum. In addition, anticancer activity were determined by MTT assay, Annexin V-FITC/PI and cell cycle analysis.

Green synthesis of silver nanoparticles from sodium para-hydroxybenzoate tetrahydrate and its apoptotic induces effect on human colon cancer cell lines.Figure optionsDownload as PowerPoint slide