کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1428129 1509167 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biocompatible surgical meshes based on decellularized human amniotic membrane
ترجمه فارسی عنوان
مش های جراحی بیو سازگار بر اساس غشای آمنیوتیک انسانی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


• A technique to decellularize HAM effectively was established.
• Biodegradable and biocompatible dHAM/PU/dHAM hamburg composite was fabricated.
• The in vivo tests demonstrated that the composite was feasible for clinic application.

Meshes play important roles to repair human tissue defect. In this work, human amniotic membrane (HAM) was decellularized and explored the efficacy as an implantable biological mesh. Surfactant, hypertonic saline, lipase and DNAase were used individually or collectively to remove all cell components and remain the extracellular matrix. Results of H&E and DAPI staining demonstrated that the method of surfactant and lipase combining with DNAase is the most effective treatment for HAM decellularization. Primary smooth muscle cells were seeded to evaluate the decellularized HAM's (dHAM) in vitro cytocompatibility. The in vivo test was performed via implantation at rabbits' uterus with clinic polypropylene mesh (PP) as the control. The results indicated that dHAM possessed good biocompatibility and will be a potential candidate for biological mesh.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 54, 1 September 2015, Pages 112–119
نویسندگان
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