Bioprocess intensification of antibiotic production by Streptomyces coelicolor A3(2) in micro-porous culture

• Preparation of novel micro-porous matrix with different physiochemical proprieties
• S. coelicolor A3(2) was cultured in those micro-porous and antibiotics was enhanced.
• Matrix pore sizes and surface chemistry influenced bacterial signalling.
• Bacterial signalling has a profound effect in the overproduction of Prodigiosin and actinorhodin.
• Prodigiosin and actinorhodin production within micro-porous was 5–17 times higher compared with liquid growth.

A novel functionalized micro-porous matrix was developed with well-controlled physicochemical proprieties such as pore size and surface chemistry. The matrix was used as a solid support in the growth of “Streptomyces coelicolor” A3(2) to enhance the production of antibiotics. The results shown support a higher production of prodigiosin and actinorhodin with overall production increase of 2–5 and 6–17, respectively, compared to conventional submerged liquid culture, offering a potential improvement in volumetric productivity.Scanning Electron Microscopy was used to evaluate pore size as well as bacterial adhesion, penetration, proliferation and migration within the micro-porous matrix.

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