کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1428247 1509172 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Repressive effects of oat extracts on intracellular lipid-droplet formation in adipocytes and a three-dimensional subcutaneous adipose tissue model
ترجمه فارسی عنوان
اثرات سرکوب کننده عصاره های جو در شکل گیری چربی های داخل سلولی در آدیپوسیت ها و یک مدل بافت چربی زیر جلدی سه بعدی
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


• We assessed the repressive effect of oat extracts on lipid droplet formation in vitro.
• The oat extracts prevented huge aggregates of lipid droplets in dermis of 3D-adipogenic models.
• The oat extracts suppressed lipid droplet formation in OP9 cells during differentiation.
• In HaCaT keratinocytes, the oat extracts prevented cell death against oxidative stress.
• The water-soluble components of oat extracts have a potential to prevent adipogenesis.

We assessed the repression of lipid-droplet formation in mouse mesenchymal stromal preadipocytes OP9 by specified oat extracts (Hatomugi, Coix lacryma-jobi var. ma-yuen) named “SPH” which were proteolytically and glucosyl-transferredly prepared from finely-milled oat whole-grain. Stimulation of OP9 preadipocytes with insulin-containing serum-replacement promoted differentiation to adipocytes, concurrently with an increase in the intracellular lipid droplets by 51.5%, which were repressed by SPH-bulk or SPH-water-extract at 840 ppm, to 33.5% or 46.9%, respectively, but not by SPH-ethanol-extract at the same dose, showing the hydrophilic property of the anti-adipogenetic ingredients. The intracellular lipid droplets were scanty for intact preadipocytes, small-sized but abundant for the SPH-unadministered adipocytes, and large-sized but few for SPH-bulk-administered adipocytes being coexistent with many lipid-droplet-lacking viable cells, suggesting “the all-or-none rule” for lipid-droplet generation in cell-to-cell. Hydrogen-peroxide-induced cell death in human epidermal keratinocytes HaCaT was prevented by SPH-bulk at 100 or 150 ppm by 5.6–8.1%, being consistent with higher viabilities of SPH-bulk-administered OP9 cells, together with repressions of both cell shrinkage and cell detachment from the culture substratum. In three-dimensional subcutaneous adipose tissue models reconstructed with HaCaT-keratinocytes and OP9-preadipocytes, lipid droplets were accumulated in dermal OP9-cell-parts, and repressed to 43.5% by SPH-bulk at 840 ppm concurrently with marked diminishment of huge aggregates of lipid droplets. Thus SPH-bulk suppresses adipogenesis-associated lipid-droplet accumulation during differentiation of OP9 preadipocytes together with lowered cytotoxicity to either HaCaT keratinocytes or the preadipocytes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 49, 1 April 2015, Pages 269–273
نویسندگان
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