کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1428308 1509181 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
BMP2-encapsulated chitosan coatings on functionalized Ti surfaces and their performance in vitro and in vivo
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
BMP2-encapsulated chitosan coatings on functionalized Ti surfaces and their performance in vitro and in vivo
چکیده انگلیسی


• The functionalization of Ti surfaces improves the adhesion of chitosan coatings.
• The BMP2-encapsulated chitosan coatings realize the effective BMP2 immobilization.
• Chitosan coatings can release BMP2 in a sustained manner.
• In vitro and in vivo studies prove that BMP2-CS-Ti has favorable osteoinductivity.

Bone morphogenic protein-2 (BMP2)-encapsulated chitosan (CS) coatings were prepared to immobilize BMP2 on titanium (Ti) surfaces. The Ti substrates were functionalized through a three-step process: alkali treatment, silanization with 3-aminopropyltriethoxysilane and aldehydation with glutaraldehyde (GA). BMP2-encapsulated CS coatings (BMP2-CS) were bonded to Ti surfaces through reactions between the aldehyde groups of GA and the amine groups of CS. Direct BMP2 immobilization on aldehyde-treated Ti (BMP2-Ti) and pure CS coatings (CS-Ti) were used as controls. The release rate of BMP2-CS-Ti was half of that of BMP2-Ti at initial stage, which indicates that the CS coatings are suitable carriers for sustained BMP2 release. The osteoinductivities of BMP2-CS-Ti, BMP2-Ti, CS-Ti and pristine Ti were examined by both in vitro cell tests and in vivo experiments. Bone marrow stem cell (BMSC) culture indicated that BMP2-CS-Ti is more potent in stimulating the differentiation of the adhering BMSC than the three other groups. Rabbit femur implantation revealed the excellent osteoinductivity of BMP2-CS-coated Ti implants. These results demonstrate that the BMP2-encapsulated CS coatings are stable osteoinductive coatings that realize the sustained release of BMP2 and maintain the activity of the protein.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 40, 1 July 2014, Pages 1–8
نویسندگان
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