کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1428334 1509181 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A study on the long term effect of biofilm produced by biosurfactant producing microbe on medical implant
ترجمه فارسی عنوان
مطالعهی اثر طولانی مدت بیوفیلم تولید شده توسط میکروب تولید کننده بیوسورفکتانت بر روی ایمپلنت پزشکی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


• Early P.aeru biofilm had genes needed for motility but later for housekeeping.
• Early B. sub biofilm had genes needed for its formation but later for maturity.
• Cells and matrix components in B. sub biofilm are higher than in P.aeru.
• Compositions of these two biofilms are different.
• So they need diverse cleaning strategies.

Low density polyethylene (LDPE) is used as a long term medical implant. Biofilm forming ability of two pathogenic microorganisms, namely, Bacillus subtilis (B. subtilis) and Pseudomonas aeruginosa (P. aeruginosa) on this polymer and the differences in the properties of these matrices are studied for a year. There are very few long term studies on biofilms formed on medical implants. After three months, colonies of B. subtilis were two times higher when compared to those of P. aeruginosa. And at the end of one year, they were two orders of magnitude higher than the later. The exopolysaccharide (EPS) and biosurfactant recovered from the polymer surface after three months were 21 and 10.4 μg/cm2 for B. subtilis and 13 and 8.6 μg/cm2 for P. aeruginosa. After one year, these were higher in B. subtilis (50 and 37.1 μg/cm2, respectively) than in P. aeruginosa (34.1 and 31.8 μg/cm2, respectively). B. subtilis consisted of protein controlling the community and sporulation development, while P. aeruginosa had either housekeeping or metabolic proteins. The EPS in the respective biofilm consisted of biosurfactants produced by B. subtilis (surfactins, m/z = 1029 to 1134) and P. aeruginosa (rhamnolipids, m/z = 568 to 705). Thermogravimetric analysis indicated that LDPE incubated with these organisms underwent a weight loss of 4 and 3% after three months and 11.1 and 9.2% after one year, respectively at 435 °C. Laccase and manganese peroxidase were detected in the biofilm which could be involved in the degradation. The biosurfactant of these microorganisms altered the hydrophobicity of the surface, favoring their attachment and proliferation.

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 40, 1 July 2014, Pages 212–218
نویسندگان
, , ,