کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1428356 1509181 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Surface modification of polycaprolactone scaffolds fabricated via selective laser sintering for cartilage tissue engineering
ترجمه فارسی عنوان
اصلاح سطح پلیکانپرولکتون داربست ساخته شده از طریق انتخاب پانل لیزر برای مهندسی بافت غضروف
کلمات کلیدی
پخت لیزری انتخابی ژلاتین، کلاژن، کندروسیت، مهندسی بافت غضروف، پوشش غوطه وری
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


• Selective laser sintered polycaprolactone scaffolds are prepared.
• Scaffolds are surface modified through immersion coating with gelatin or collagen.
• Collagen-scaffold is the best for cartilage tissue engineering in vitro.
• Chondrocytes/collagen-scaffold reveals enhanced cartilage tissue formation in vivo.

Surface modified porous polycaprolactone scaffolds fabricated via rapid prototyping techniques were evaluated for cartilage tissue engineering purposes. Polycaprolactone scaffolds manufactured by selective laser sintering (SLS) were surface modified through immersion coating with either gelatin or collagen. Three groups of scaffolds were created and compared for both mechanical and biological properties. Surface modification with collagen or gelatin improved the hydrophilicity, water uptake and mechanical strength of the pristine scaffold. From microscopic observations and biochemical analysis, collagen-modified scaffold was the best for cartilage tissue engineering in terms of cell proliferation and extracellular matrix production. Chondrocytes/collagen-modified scaffold constructs were implanted subdermally in the dorsal spaces of female nude mice. Histological and immunohistochemical staining of the retrieved implants after 8 weeks revealed enhanced cartilage tissue formation. We conclude that collagen surface modification through immersion coating on SLS-manufactured scaffolds is a feasible scaffold for cartilage tissue engineering in craniofacial reconstruction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 40, 1 July 2014, Pages 389–397
نویسندگان
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