کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1428440 1509173 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Surface plasma functionalization influences macrophage behavior on carbon nanowalls
ترجمه فارسی عنوان
کاربردی سازی پلاسما سطح روی رفتار ماکروفاژ بر روی نانولوله های کربنی تأثیر می گذارد
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


• N2 and O2 plasma treatments alter the CNW surface chemistry and wettability.
• Cells seeded on CNW scaffolds are viable and metabolically active.
• Surface functional groups, independent of surface wettability, affect cell response.
• O2 plasma treatment of CNW leads to a more activated macrophage phenotype.

The surfaces of carbon nanowall samples as scaffolds for tissue engineering applications were treated with oxygen or nitrogen plasma to improve their wettability and to functionalize their surfaces with different functional groups. X-ray photoelectron spectroscopy and water contact angle results illustrated the effective conversion of the carbon nanowall surfaces from hydrophobic to hydrophilic and the incorporation of various amounts of carbon, oxygen and nitrogen functional groups during the treatments. The early inflammatory responses elicited by un-treated and modified carbon nanowall surfaces were investigated by quantifying tumor necrosis factor-alpha and macrophage inflammatory protein-1 alpha released by attached RAW 264.7 macrophage cells. Scanning electron microscopy and fluorescence studies were employed to investigate the changes in macrophage morphology and adhesive properties, while MTT assay was used to quantify cell proliferation. All samples sustained macrophage adhesion and growth. In addition, nitrogen plasma treatment was more beneficial for cell adhesion in comparison with un-modified carbon nanowall surfaces. Instead, oxygen plasma functionalization led to increased macrophage adhesion and spreading suggesting a more activated phenotype, confirmed by elevated cytokine release. Thus, our findings showed that the chemical surface alterations which occur as a result of plasma treatment, independent of surface wettability, affect macrophage response in vitro.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 48, 1 March 2015, Pages 118–125
نویسندگان
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