کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1428496 1509173 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dewetting based fabrication of fibrous micro-scaffolds as potential injectable cell carriers
ترجمه فارسی عنوان
ساخت میکرو داربست های فیبر دار به عنوان یک حامل سلول های تزریقی امکان پذیر است
کلمات کلیدی
میکرو سازنده فیبر، ساختارهای تزریقی، احتباس سلولی، از بین بردن مهندسی و بازسازی بافت
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


• Fibrous micro-scaffolds fabricated by dewetting of polymer thin films
• Micro-scaffolds by dewetting formed with proper conditions/material properties.
• Cells attached/grew on micro-scaffolds yielded cell/polymer micro-constructs.
• Micro-constructs injected through a G20 needle with minimal cell loss/damage
• Enhanced cell retention achieved in both in vitro and in vivo injection studies

Although regenerative medicine utilizing tissue scaffolds has made enormous strides in recent years, many constraints still hamper their effectiveness. A limitation of many scaffolds is that they form surface patches, which are not particularly effective for some types of “wounds” that are deep within tissues, e.g., stroke and myocardial infarction. In this study, we reported the generation of fibrous micro-scaffolds feasible for delivering cells by injection into the tissue parenchyma. The micro-scaffolds (widths < 100 μm) were made by dewetting of poly(lactic-co-glycolic acid) thin films containing parallel strips, and cells were seeded to form cell/polymer micro-constructs during or post the micro-scaffold fabrication process. Five types of cells including rat induced vascular progenitor cells were assessed for the formation of the micro-constructs. Critical factors in forming fibrous micro-scaffolds via dewetting of polymer thin films were found to be properties of polymers and supporting substrates, temperature, and proteins in the culture medium. Also, the ability of cells to attach to the micro-scaffolds was essential in forming cell/polymer micro-constructs. Both in vitro and in vivo assessments of injecting these micro-scaffolding constructs showed, as compared to free cells, enhanced cell retention at the injected site, which could lead to improved tissue engineering and regeneration.

Dewetting based generation of cell/polymer micro-constructs allowed dramatically enhanced cell retention/survival as compared to free cells when injected.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 48, 1 March 2015, Pages 663–672
نویسندگان
, , , ,