Development of a highly sensitive MIP based-QCM nanosensor for selective determination of cholic acid level in body fluids

• The purpose is to synthesize a new cholic acid imprinted QCM nanosensor by MIP.
• Analytical applications of QCM nanosensor were investigated.
• The cholic acid levels in body fluids were determined by prepared QCM nanosensor.

Determination of cholic acid is very important and necessary in body fluids due to its both pharmaceutical and clinical significance. In this study, a quartz crystal microbalance (QCM) nanosensor, which is imprinted cholic acid, has been developed for the assignation of cholic acid. The cholic acid selective memories have been generated on QCM electrode surface by using molecularly imprinted polymer (MIP) based on methacryloylamidohistidine-copper (II) (MAH-Cu(II)) pre-organized monomer. The cholic acid imprinted nanosensor was characterized by atomic force microscopy (AFM) and then analytical performance of the cholic acid imprinted QCM nanosensor was studied. The detection limit was found to be 0.0065 μM with linear range of 0.01–1000 μM. Moreover, the high value of Langmuir constant (b) (7.3 * 105) obtained by Langmuir graph showed that the cholic acid imprinted nanosensor had quite strong binding sites affinity. At the last step of this procedure, cholic acid levels in body fluids were determined by the prepared imprinted QCM nanosensor.

QCM responses of the cholic acid imprinted and non-imprinted nanosensors (CCA = 0.1 μM).Figure optionsDownload as PowerPoint slide