EDTA assisted synthesis of hydroxyapatite nanoparticles for electrochemical sensing of uric acid

• EDTA- hydroxyapatite (HA) nanoparticles have been synthesized by microwave irradiation method.
• A novel amperometric Uric Acid biosensor has been fabricated using E-HA/GCE.
• The fabricated sensor exhibits a wide linear range, good stability and high reproducibility.
• The sensor was applied for the detection of UA in human blood serum and urine.

Hydroxyapatite nanoparticles have been synthesized using EDTA as organic modifier by a simple microwave irradiation method and its application for the selective determination of uric acid (UA) has been demonstrated. Electrochemical behavior of uric acid at HA nanoparticle modified glassy carbon electrode (E-HA/GCE) has been investigated by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), linear sweep voltammetry (LSV) and amperometry. The E-HA modified electrode exhibits efficient electrochemical activity towards uric acid sensing without requiring enzyme or electron mediator. Amperometry studies revealed that the fabricated electrode has excellent sensitivity for uric acid with the lowest detection limit of 142 nM over a wide concentration range from 1 × 10− 7 to 3 × 10− 5 M. Moreover, the studied E-HA modified GC electrode exhibits a good reproducibility and long-term stability and an admirable selectivity towards the determination of UA even in the presence of potential interferents. The analytical performance of this sensor was evaluated for the detection of uric acid in human urine and blood serum samples.

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