One-pot facile synthesis of PEGylated superparamagnetic iron oxide nanoparticles for MRI contrast enhancement

• PEGylated SPIONs are synthesized via a one-pot facile route.
• They show high relaxivity and low cytotoxicity.
• Both liver and spleen are found significantly contrast-enhanced in MR scanning.
• They can be cleared from the body via hepatobiliary processing over a period of 14 days.

Polyethylene glycol (PEG)-coated superparamagnetic iron oxide nanoparticles (PEG·SPIONs) were prepared by a facile one-pot approach. The synthesized PEG·SPIONs were found to be uniform in size with an average hydrodynamic diameter of 11.7 nm. PEG·SPIONs exhibited excellent dispersibility in water, colloidal stability, and biocompatibility. The magnetic resonance imaging (MRI) properties of PEG·SPIONs were characterized both in vitro and in vivo. The dual contrast both in T1 and T2-weighted imaging was well enhanced with longitudinal and transverse relaxivity (r1, r2) of 35.92 s− 1 per mM of Fe3 + and 206.91 s− 1 per mM of Fe3 + respectively. In vivo T2-weighted MRI shows pronounced enhancement in the liver and spleen but not in T1-weighted MRI. Accumulations of nanoparticles were found primarily in the liver, spleen, and intestine, while much lower uptake in the kidney, heart, and lungs. A gradual excretion of PEG·SPIONs was observed via hepatobiliary (HB) processing over a period of 14 days. The toxicity of PEG·SPIONs was also evaluated in vitro and in vivo. PEG·SPIONs were found to be biocompatible by investigating organ tissues after hematoxylin–eosin staining. The conclusion of the study indicates a high potential of PEG·SPIONs in medical MRI.

PEGylated superparamagnetic iron oxide nanoparticles are synthesized via a one-pot facile route. They show low cytotoxicity, and present good in vivo MRI enhancement.Figure optionsDownload as PowerPoint slide