کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1428889 987160 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of bovine serum albumin on the functionality and structure of catanionic surfactant at air–buffer interface
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Effect of bovine serum albumin on the functionality and structure of catanionic surfactant at air–buffer interface
چکیده انگلیسی

Interaction of bovine serum albumin (BSA) with the solvent spread monolayer of a catanionic surfactant, octadecyltrimethylammonium dodecylsulfate, (C18TA+DS−) at the air–buffer interface was investigated by measuring the surface pressure with time and change in surface area. Dipalmitoylphosphatidylcholine (DPPC) was used as reference. Kinetics of BSA desorption from the interface to the buffer subphase, that of C18TA+DS− and DPPC through their interaction with BSA, were also studied at different BSA concentrations (in the subphase) and surface pressures. Surface pressure (π)–area (A) isotherms (at pH = 5.4, μ = 0.01, T = 298 K) revealed that the coacervate/DPPC monolayer becomes expanded in the presence of BSA at low π while their protein bound species are released into the subphase at high π. Film morphology, studied by epifluorescence microscopy (EFM) and atomic force microscopy (AFM), reveals that the sizes of the domains of both DPPC and coacervate decrease in the presence of BSA. Presence of BSA in the coacervate and DPPC monolayer was supported from AFM data analysis.

Figure optionsDownload as PowerPoint slideHighlights
► Effect of BSA on the functionality and structure of C18TA+DS−/DPPC at the air–buffer interface was studied.
► BSA molecules coadsorb at lower surface pressure, while they abstract amphiphiles at higher surface pressure into the bulk.
► Kinetic studies of adsorption/desorption of BSA at/from the interface were performed.
► Organized amphiphiles are perturbed in the presence of BSA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 33, Issue 2, 1 March 2013, Pages 836–843
نویسندگان
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