Ligand exchange based paraoxon imprınted QCM sensor

In the present work, a paraoxon imprinted QCM sensor has been developed for the determination of paraoxon based on the modification of paraoxon imprinted film onto a quartz crystal combining the advantages of high selectivity of the piezoelectric microgravimetry using MIP film technique and high sensitivity of QCM detection. The paraoxon selective memories have formed on QCM electrode surface by using a new metal–chelate interaction based on pre-organized monomer and the paraoxon recognition activity of these molecular memories was investigated. Molecular imprinted polymer (MIP) film for the detection of paraoxon was developed and the analytical performance of paraoxon imprinted sensor was studied. The molecular imprinted polymer were characterized by FTIR measurements. Paraoxon imprinted sensor was characterized with AFM and ellipsometer. The study also includes the measurement of binding interaction of paraoxon imprinted quartz crystal microbalance (QCM) sensor, selectivity experiments and analytical performance of QCM electrode. The detection limit and the affinity constant (Kaffinity) were found to be 0.06 μM and 2.25 × 104 M− 1 for paraoxon [MAAP–Cu(II)–paraoxon] based thin film, respectively. Also, it has been observed that the selectivity of the prepared paraoxon imprinted sensor is high compared to a similar chemical structure which is parathion.

Figure shows the AFM image of [(MAAP)2–Cu–paraoxon] coated gold electrodes. We have proposed a novel phosphotriesterase mimick surface imprinted polymeric QCM sensor for selectively determination of the nerve agent by MAAP–Cu(II) as a new metal-chelating monomer via metal coordination–chelation interactions and paraoxon (diethyl-4-nitrophenyl phosphate, target nevre agent) templates. The paraoxon selective memories were formed on QCM electrode surface by using a new metal–chelate interaction based on pre-organized monomer and paraoxon recognition activity of these molecular memories was investigated.Figure optionsDownload as PowerPoint slideHighlights
► We have designed to MIP/QCM system for paraoxon determination.
► We have proposed molecular imprinted polymer (MIP) film for the detection of paraoxon.
► We have investigated measurement of binding interaction of paraoxon imprinted quartz crystal microbalance (QCM) sensor.