کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1428991 1509184 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Surface functionalization of nanoporous alumina with bone morphogenetic protein 2 for inducing osteogenic differentiation of mesenchymal stem cells
ترجمه فارسی عنوان
کاربردی بودن سطح آلومینای نانوپور با پروتئین مورفوژنیک استخوانی 2 برای القای تمایز استئوژنیک سلول های بنیادی مزانشیمی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


• BMP2 was immobilized onto nanoporous alumina substrates with different pore sizes.
• BMP2-immobilized substrates were able to promote adhesion and spreading of MSCs.
• BMP2-immobilized substrates were favorable for cell growth of MSCs.
• BMP2-immobilized substrates promoted osteogenic differentiation of MSCs.

Many studies have demonstrated the possibility to regulate cellular behavior by manipulating the specific characteristics of biomaterials including the physical features and chemical properties. To investigate the synergistic effect of chemical factors and surface topography on the growth behavior of mesenchymal stem cells (MSCs), bone morphorgenic protein 2 (BMP2) was immobilized onto porous alumina substrates with different pore sizes. The BMP2-immobilized alumina substrates were characterized with scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). Growth behavior and osteogenic differentiation of MSCs cultured on the different substrates were investigated. Cell adhesion and morphological changes were observed with SEM, and the results showed that the BMP2-immobilized alumina substrate was able to promote adhesion and spreading of MSCs. MTT assay and immunofluorescence staining of integrin β1 revealed that the BMP2-immobilized alumina substrates were favorable for cell growth. To evaluate the differentiation of MSCs, osteoblastic differentiation markers, such as alkaline phosphatase (ALP) activity and mineralization, were investigated. Compared with those of untreated alumina substrates, significantly higher ALP activities and mineralization were detected in cells cultured on BMP2-immobilized alumina substrates. The results suggested that surface functionalization of nanoporous alumina substrates with BMP2 was beneficial for cell growth and osteogenic differentiation. With the approach of immobilizing growth factors onto material substrates, it provided a new insight to exploit novel biofunctional materials for tissue engineering.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 37, 1 April 2014, Pages 120–126
نویسندگان
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