Bioelectrode for detection of human salivary amylase

The aim of this study is the development of a novel bioelectrode based on the immobilization of a specific antibody for salivary amylase onto a graphite electrode modified with poly(3-hydroxyphenylacetic) acid. For this purpose, human salivary alpha-amylase was applied to an immunoaffinity-purified anti-alpha-amylase polyclonal antibody. The bioelectrode was incubated with salivary amylase or lysozyme (interfering salivary), and the interaction between the antibody and the enzymes was analyzed through electrochemical impedance spectroscopy. The results indicated the specificity of the bioelectrode to salivary alpha-amylase. Therefore, the combination of the graphite electrode with poly(3-hydroxyphenylacetic acid) appears to be a promising strategy for antigen immobilization and other biological recognition elements, thus presenting the potential for the production of a label-free biochip.

► The alpha-amylase and anti-alpha-amylase polyclonal antibody were purified using human saliva.
► A novel bioelectrode was developed based on the immobilization of this specific antibody onto graphite electrode modified with poly(3-hydroxyphenylacetic) acid.
► The bioelectrode was specificity for salivary alpha-amylase.
► The graphite electrode with poly(3-hydroxyphenylacetic acid) appears to be a promising strategy for antigen immobilization.