کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1429199 987165 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cells behaviors and genotoxicity on topological surface
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Cells behaviors and genotoxicity on topological surface
چکیده انگلیسی


• Different cells behaviors on microstructure surfaces were discussed in this paper.
• The expression of cell protein of Vinculin was studied in this research.
• Cellular micronuclei test was applied to evaluate cells' genotoxicity.
• Cell genotoxicity was first studied in the research field of topological surfaces.

To investigate different cells behaviors and genotoxicity, which were driven by specific microenvironments, three patterned surfaces (pillars, wide grooves and narrow grooves) and one smooth surface were prepared by template-based technique. Vinculin is a membrane-cytoskeletal protein in focal adhesion plaques and associates with cell–cell and cell–matrix junctions, which can promote cell adhesion and spreading. The immunofluorescence staining of vinculin revealed that the narrow grooves patterned substrate was favorable for L929 cell adhesion. For cell multiplication, the narrow grooves surface was fitted for the proliferation of L929, L02 and MSC cells, the pillars surface was only in favor of L929 cells to proliferate during 7 days of cell cultivation. Cell genetic toxicity was evaluated by cellular micronuclei test (MNT). The results indicated that topological surfaces were more suitable for L929 cells to proliferate and maintain the stability of genome. On the contrary, the narrow grooves surface induced higher micronuclei ratio of L02 and MSC cells than other surfaces. With the comprehensive results of cell multiplication and MNT, it was concluded that the wide grooves surface was best fitted for L02 cells to proliferate and have less DNA damages, and the smooth surface was optimum for the research of MSC cells in vitro.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 33, Issue 6, 1 August 2013, Pages 3465–3473
نویسندگان
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