کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1429300 987168 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of the attachment, proliferation, and differentiation of osteoblast on a calcium carbonate coating on titanium surface
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Evaluation of the attachment, proliferation, and differentiation of osteoblast on a calcium carbonate coating on titanium surface
چکیده انگلیسی

Titanium has been reported to have some limitations in dental and orthopaedic clinical application. This study described a coating process using a simple chemical method to prepare calcium carbonate coatings on smooth titanium (STi) and sandblasted and acid-etched titanium (SATi), and evaluated the biological response of the materials in vitro. The surfaces of STi, SATi, calcium carbonate coated STi (CC-STi) and calcium carbonate coated SATi (CC-SATi) were characterized for surface roughness, contact angles, surface morphology and surface chemistry. The morphology of MG63 cells cultured on the surfaces was observed by SEM and Immuno-fluorescence staining. Cell attachment/proliferation was assessed by MTT assay, and cell differentiation was evaluated by alkaline phosphatase (ALP) activity. MG63 was found to attach favorably to calcium carbonate crystals with longer cytoplasmic extensions on CC-STi and CC-SATi, resulting in lower cell proliferation but higher ALP activity when compared to STi and SATi respectively. Moreover, CC-SATi is more favorable than CC-STi in terms of biological response. In conclusion, the calcium carbonate coatings on titanium were supposed to improve the osteointegration process and stimulate osteoblast differentiation, especially in early stage. And this method could possibly be a feasible alternative option for future clinical application.


► Calcium carbonate coatings were prepared on titanium substrates.
► The coating process is simple and cost-effective.
► Calcium carbonate coating could induce differentiation toward an osteoblastic phenotype.
► Calcium carbonate coating could enhance the osteointegration process especially in early stage.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 31, Issue 5, 20 July 2011, Pages 1055–1061
نویسندگان
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