کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1429326 987170 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biocompatibility evaluation of magnetosomes formed by Acidithiobacillus ferrooxidans
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Biocompatibility evaluation of magnetosomes formed by Acidithiobacillus ferrooxidans
چکیده انگلیسی

Magnetite nanocrystal has been extensively used in biomedical field. Currently, an interesting alternative to synthetic magnetic Fe3O4 nanoparticles, called magnetosome, has been found in magnetotactic bacteria. It has been reported that Acidithiobacillus ferrooxidans (At. ferrooxidans) has a potential to synthesize magnetosome. In this study, transmission electron microscope (TEM) was used to analyze the magnetite particles in At. ferrooxidans BY-3. The magnetosomes formed by this bacterium were isolated by a method combining ultracentrifugation and magnetic separation. Crystalline phase and surface functional group of the magnetosomes were investigated by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), respectively. Biocompatibility of the magnetosomes was systematically evaluated at various concentrations (0.5, 1.0, 2.0 and 4.0 mg/ml). MTT test, hemolysis assay and Micronucleus Test were carried out to evaluate in vitro cytotoxicity, blood toxicity and genotoxicity of magnetosomes, respectively. Under these conditions, magnetosomes showed no cytotoxic, genotoxic and hemolytic effects up to 4.0 mg/ml indicating good biocompatibility of these biological nanoparticles. These revealed that the magnetosomes might have a potential for biotechnological and biomedical applications in the future.


► The production of magnetosomes from At. ferrooxidans has been easily available.
► Several techniques are used to characterize properties of the magnetosomes.
► The magnetosomes have no cytotoxicity, no hemolysis activity and no genotoxicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 32, Issue 7, 1 October 2012, Pages 1802–1807
نویسندگان
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