کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1429327 987170 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sensitive DNA hybridization detection based on the deposition of gold nanoparticle on lyophobic surface
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Sensitive DNA hybridization detection based on the deposition of gold nanoparticle on lyophobic surface
چکیده انگلیسی

An assay for the detection of DNA hybridization was developed using Ellipsometric Scanner (ES) as a detection method. The different deposition trends of Au nanoparticle on glutaraldehyde-covered Si substrate caused by the different electrostatic properties of Au nanoparticles after interaction with ssDNA and dsDNA respectively can be observed by measuring the ellipsometric parameter y. At the same time, the average height of the y with respect to the cross-sectional contour c can be described by y = 0.0920 + 2.43c in the range of 5.4 to 27 nM (r = 0.943, c is target concentration, the unit is μM). This assay using ES imaging could successfully detect the total amounts of the target as low as 5.4 fmol. The proposed assay system has the advantage of allowing label-free detection, high sensitivity, and operational simplicity.


► An assay for the detection of DNA hybridization was developed using Ellipsometric Scanner (ES) as a detection method.
► This assay using ES imaging could successfully detect the total amounts of the target as low as 5.4 fmol.
► The proposed assay system has the advantage of allowing label-free detection, high sensitivity, and operational simplicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 32, Issue 7, 1 October 2012, Pages 1808–1812
نویسندگان
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