کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1429410 987174 2013 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reducing Staphylococcus aureus biofilm formation on stainless steel 316L using functionalized self-assembled monolayers
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Reducing Staphylococcus aureus biofilm formation on stainless steel 316L using functionalized self-assembled monolayers
چکیده انگلیسی

Stainless steel 316L (SS316L) is a common material used in orthopedic implants. Bacterial colonization of the surface and subsequent biofilm development can lead to refractory infection of the implant. Since the greatest risk of infection occurs perioperatively, strategies that reduce bacterial adhesion during this time are important. As a strategy to limit bacterial adhesion and biofilm formation on SS316L, self-assembled monolayers (SAMs) were used to modify the SS316L surface. SAMs with long alkyl chains terminated with hydrophobic (− CH3) or hydrophilic (oligoethylene glycol) tail groups were used to form coatings and in an orthogonal approach, SAMs were used to immobilize gentamicin or vancomycin on SS316L for the first time to form an “active” antimicrobial coating to inhibit early biofilm development. Modified SS316L surfaces were characterized using surface infrared spectroscopy, contact angles, MALDI-TOF mass spectrometry and atomic force microscopy. The ability of SAM-modified SS316L to retard biofilm development by Staphylococcus aureus was functionally tested using confocal scanning laser microscopy with COMSTAT image analysis, scanning electron microscopy and colony forming unit analysis. Neither hydrophobic nor hydrophilic SAMs reduced biofilm development. However, gentamicin-linked and vancomycin-linked SAMs significantly reduced S. aureus biofilm formation for up to 24 and 48 h, respectively.


► SS316L was modified with glycol terminated SAMs in order to reduce biofilm growth.
► Antibiotics gentamicin and vancomycin were immobilized on SS316L via SAMs.
► Only the antibiotic modifications reduced biofilm development on SS316L.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 33, Issue 4, 1 May 2013, Pages 2059–2069
نویسندگان
, , , , , , ,