Spectrophotometric detection of histidine and lysine using combined enzymatic reactions

• Specific interaction of ARS for amino acid was applied to amino acid sensing.
• Range from 1.5 to 55 μM of histidine was measured by the sensing method.
• The biosensing system for histidine showed selective response to histidine.
• Lysine could be also measured selectively in this method.

An amino acid-sensing system with absorption spectrophotometric detection was developed. To ensure specific recognition of each amino acid, aminoacyl-tRNA synthetases were employed and the concentration of NADH produced by way of several enzymatic reactions was measured. Using this detection system, from 1.5 to 55 μM of histidine and from 15 to 95 μM of lysine could be measured selectively in HEPES-KOH buffer (pH 8.0).