کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1429583 | 987176 | 2012 | 6 صفحه PDF | دانلود رایگان |
Porous scaffolds play important roles in tissue engineering. Biodegradable synthetic polymers, such as poly(l-lactic acid) (PLLA), frequently are used in the preparation of porous scaffolds. Pretreating the surface of a PLLA porous scaffold is required to increase its wettability for smooth cell seeding due to the hydrophobic property of the scaffold's surface. In this study, a simple coating method was used to modify the surface of the PLLA sponges. The coating method included three steps: filling the PLLA sponge pores with collagen aqueous solution, centrifuging to remove excess collagen, and, finally, freeze-drying. Compared with the uncoated PLLA sponge, the collagen-coated PLLA sponge demonstrated both improved wettability and high water absorption. Cells were smoothly seeded in the collagen-coated PLLA sponges by dropping a cell suspension solution onto the sponges. Cells adhered to the collagen-coated sponge and were distributed homogeneously throughout the collagen-coated PLLA sponge.
► A simple collagen-coating method is used to modify surface of PLLA sponges.
► Surface of collagen-coated PLLA sponges becomes hydrophilic.
► Collagen coating enables easy cell seeding.
► Cells penetrate and distribute homogeneously in collagen-coated PLLA sponges.
Journal: Materials Science and Engineering: C - Volume 32, Issue 2, 1 March 2012, Pages 290–295