کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1429657 987177 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of chlorpyriphos in broccoli using a voltammetric acetylcholinesterase sensor based on carbon nanostructure–chitosan composite material
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Determination of chlorpyriphos in broccoli using a voltammetric acetylcholinesterase sensor based on carbon nanostructure–chitosan composite material
چکیده انگلیسی

The widespread use of organophosphate pesticides (OPPs) in agriculture leads to residue accumulation in the environment, which is dangerous to human health and disrupts the ecological balance. This study reports results obtained by a standard chromatographic method (high performance liquid chromatography, HPLC) and an amperometric method for chlorpyriphos (CPF) determination in broccoli. Reversed-phase HPLC with UV–VIS detection was used for the separation, and the mobile phase was acetonitrile–water (75:25 v/v). The electrochemical experiments were performed in phosphate buffer solutions at pH = 7.4, with an incubation time of 10 min. The response of the sensor was a linear function of CPF concentration from 10− 10 to 10− 7 M with a corresponding equation: y = 0.1467x + 1.4472 (R2 = 0.9959) and a detection limit of 1.58 × 10− 10 M. The biosensor methodology was used to analyze CPF directly in broccoli that had been previously spiked with CPF solution.


► The first attempt to use graphite nanoplatelets in this type of biosensor.
► The first attempt to develop a method for rapid detection of chloropyriphos based on this sensor.
► The sensor has a good detection limit of 10− 10 M for chloropyrifos in broccoli.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 32, Issue 4, 1 May 2012, Pages 1001–1004
نویسندگان
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