Amperometric glucose biosensor based on in situ electropolymerized polyaniline/poly(acrylonitrile-co-acrylic acid) composite film

A new type of in situ electropolymerization was used for electrochemical biosensor design. The biologic film was prepared by in situ electropolymerization of aniline into microporous poly(acrylonitrile-co-acrylic acid)-coated platinum electrode in the presence of glucose oxidase. The results of EIS and SEM indicated the successful immobilization for enzyme in the composite polymer film. The novel glucose biosensor exhibited good selectivity and operational stability, which showed no apparent loss of activity after 40 consecutive measurements and intermittent usage for 45 days with storage in a phosphate buffer solution at 4°C. In addition, optimization of the biosensor construction as well as effects of applied potential, pH value of solution, temperature and common interfering compounds on the amperometric response of the sensor were investigated and discussed.