Integrated microanalytical system based on electrochemical detection and cell positioning

The development and preliminary evaluation of an integrated microanalytical system based on electrochemical detection and cell positioning is reported here. The chip includes ion-sensitive field effect transistors (ISFET), a single pair of castellated microelectrodes and a reference Ag/AgCl microelectrode. The ISFETs have shown good operating characteristics, with a 52 mV/pH response. Yeast cells and bacteria have been positioned on the gate region of the ISFET sensor using single pair of castellated microelectrodes.