کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1431059 1509189 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical study of human olfactory receptor OR 17–40 stimulation by odorants in solution
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Electrochemical study of human olfactory receptor OR 17–40 stimulation by odorants in solution
چکیده انگلیسی

The human olfactory receptor OR 17–40 co-expressed with α-subunit of Golf protein in yeast was attempted as a bio recognition part of impedimetric biosensor. The receptor in its natural membrane environment was anchored to a gold-coated glass substrate modified with thiol-based multilayer. Step-by-step building-up of the OR 17–40 based biofilm was monitored using surface plasmon resonance technique. Stimulation of the OR 17–40 with its cognate odorant helional in phosphate-buffered saline was probed by means of electrochemical impedance spectroscopy under various conditions. Activation of OR 17–40 in the presence of GTP-γ-S at 4 °C was found to improve the sensitivity of the developed label-free biosensor, probably via the enhancement of the specific biochemical signal.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 28, Issues 5–6, 1 July 2008, Pages 633–639
نویسندگان
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