The use of novel photobleachable phthalocyanines to image DNA

The interaction of cationic photobleachable cobalt 1 and palladium 2 phthalocyanines (pcs) with calf thymus (CT) DNA was investigated by UV/vis spectrophotometric methods and gel electrophoresis. With the addition of pcs, the change in the thermal denaturation profile of DNA was observed. The results indicated that these molecules exhibit efficient DNA binding activity. The new cationic cobalt derivative 1 was characterized by elemental analysis, UV, IR and mass spectrophotometry.

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► In this study a new CoPc and its quaternized derivative (1) was synthesized.
► Binding of compound (1) and (2) to CT-DNA was proved by UV/vis spectra, Thermal denaturation profiles and gel electrophoresis.
► Water soluble nature of (1) and (2) makes these compounds beneficial and preferable agents to be used as novel dyes for biological studies with the less harmful property.